The Subway will be closed on two Sundays in July and August.
The shutdown is to allow new trains to be tested before they are put into service.
SPT has announced the Subway will close all day on July 30 and August 20.
Stations will be closed and no trains will run for passengers on the affected days.
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The new trains need to complete safety checks on the tracks before they can be used to ensure they are fault free.
The trains must run on the system for 2000 miles without fault, which is more than 300 laps of the subway’s 15 stations.
Once complete the manufacturer, Stadler, will officially hand them over to SPT ahead of their introduction to passenger service later this year.
Mark Toner, SPT Head of Engineering, said: “This is the last stage in the critical train testing programme before the new trains can be introduced into passenger service.
“While the trains have been thoroughly tested both off-site at our dedicated train track and in the system at night, they now have to complete 2000 miles in the system, that’s about 308 times round the Subway, fault-free.
“I know Subway users have been very keen to see the new trains in service, as have we, so this is a very exciting time for everyone involved.”
A shuttle bus service will operate between Govan Subway Station and Partick Interchange, to allow connections with Scotrail services and bus services.
If a train develops a fault during the fault-free running, it is removed from the system while the fault is fixed and the clock resets on the 2000 miles so the process does take some time.
More Sunday closures are expected later in the year to allow checks on the signalling and communication system for the new trains.
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