Douglas Ross has said he will bring a motion of no confidence in Humza Yousaf.
The move follows the First Minister ending the Bute House agreement and sacking Patrick Harvie and Lorna Slater as government ministers.
It means Yousaf will now lead a minority government with 63 MSPs, two short of the required majority.
READ NEXT: Tories to bring motion of no confidence in Humza Yousaf
He will rely on votes from other parties to get legislation through and to see off a vote of no confidence.
What happens next depends on whether the Conservative motion gets backing of other MSPs
If Douglas Ross’s motion gets the support of 25 MSPs it will trigger a debate and a vote in the Scottish Parliament.
For the motion to be passed in the first minister it requires a simple majority of MSPs voting.
That means Ross would need the backing of Labour, Lib Dems and the Greens and the one Alba MSP to succeed.
It doesn’t automatically mean Humza Yousaf would resign as First Minister.
If there is a vote of no-confidence in the Government - both the first minister and all ministers are required to resign.
Even then there would not necessarily be an election.
In that case, the Parliament is not automatically dissolved, instead the Parliament has 28 days to choose a new first minister. If it cannot do so, Parliament is dissolved and an election would take place.
A Scottish Parliament spokesperson stated: “A simple majority in relation to votes of no confidence in Ministers or in the Government requires only the number of members voting 'for' to be greater than those 'against' - no account is taken of any members who abstain.
“If there is a vote of no-confidence in the First Minister it would be up to the First Minister how they responded once the will of the Parliament had been made clear.”
“If MSPs vote to pass a no confidence motion about all Scottish ministers, it could lead to a Scottish Parliament election.”
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